Correction to ‘Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity’
نویسندگان
چکیده
These authors contributed equally to this study. Present address: Institut National de la Recherche Agronomique, Metagenopolis (US1367), 78350 Jouy-en-Josas, France. Present address: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Béjaia, Algeria. jjPresent address: CEA, CNRS, Univ EVRY, Université Paris-Saclay, Génomique Métabolique (UMR8030), Laboratoire iSSB, Génopole Campus 1, Genavenir 6, 5 rue Henri Desbruères, 91030 Evry, France.
منابع مشابه
Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity
During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase briefly extends RNA primers synthesized by the primase DnaG prior to handing-off DNA synthesis to PolC. Here, we show in vivo that (i) the polym...
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Bacillus subtilis has two replicative DNA polymerases. PolC is a processive high-fidelity replicative polymerase, while the error-prone DnaEBs extends RNA primers before hand-off to PolC at the lagging strand. We show that DnaEBs interacts with the replicative helicase DnaC and primase DnaG in a ternary complex. We characterize their activities and analyse the functional significance of their i...
متن کاملTwo essential DNA polymerases at the bacterial replication fork.
DNA replication in bacteria is carried out by a multiprotein complex, which is thought to contain only one essential DNA polymerase, specified by the dnaE gene in Escherichia coli and the polC gene in Bacillus subtilis. Bacillus subtilis genome analysis has revealed another DNA polymerase gene, dnaE(BS), which is homologous to dnaE. We show that, in B. subtilis, dnaE(BS) is essential for cell v...
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Firmicutes have two distinct replicative DNA polymerases, the PolC leading strand polymerase, and PolC and DnaE synthesizing the lagging strand. We have reconstituted in vitro Bacillus subtilis bacteriophage SPP1 θ-type DNA replication, which initiates unidirectionally at oriL. With this system we show that DnaE is not only restricted to lagging strand synthesis as previously suggested. DnaG pr...
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Complex viruses that encode their own initiation proteins and subvert the host's elongation apparatus have provided valuable insights into DNA replication. Using purified bacteriophage SPP1 and Bacillus subtilis proteins, we have reconstituted a rolling circle replication system that recapitulates genetically defined protein requirements. Eleven proteins are required: phage-encoded helicase (G4...
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عنوان ژورنال:
دوره 7 شماره
صفحات -
تاریخ انتشار 2017